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KMID : 0914820030030020084
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Journal of the Korean Gastric Cancer Association
2003 Volume.3 No. 2 p.84 ~ p.87
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Mutational Analysis of Proapoptotic Bcl-2 Family Members in Gastric Carcinomas
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Yoo Nam-Jin
Lee Jong-Woo
Song Young-Hwa
Kim Hong-Sug
Park Won-Sang
Lee Jung-Yong
Lee Sug-Hyung
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Abstract
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Purpose: Evidence exists that dysregulation of Bcl-2 family members is involved in the pathogenesis of cancer development. The aim of this study was to explore whether the somatic mutation of proapoptotic Bcl-2 member genes, one of the mechanisms that prolong the survival of cancer cells, is involved in gastric carcinogenesis.
Materials and Methods: In the current study, to detect somatic mutations of the DNA sequences encoding the Bcl-2 homology 3 (BH3) domain of the human BAD, BIM, BIK, and Bcl-G genes in 60 advanced gastric adenocarcinomas, we used the polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), and DNA sequencing.
Results: The SSCP analysis revealed no mutations in the coding regions of the BH3 domain in the cancers.
Conclusion: The data presented here indicate that proapoptotic Bcl-2 member genes, BAD, BIM, BIK, and Bcl-G, may not be mutated in human gastric carcinomas and suggest that these genes might be altered by mechanisms other mechanisms somatic mutation.
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KEYWORD
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Gastric carcinoma, BAD, BIM, BIK, Bcl-G, Apoptosis, Mutation
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